spei hf (New England Biolabs)
96
Structured Review
New England Biolabs
spei hf
Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spei+hf/SpeI-HF/pmc13129541-159-17-18
Average 96 stars, based on 1132 article reviews
Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spei+hf/SpeI-HF/pmc13129541-159-17-18
Average 96 stars, based on 1132 article reviews
spei hf - by Bioz Stars,
2026-10
96/100 stars
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Plasmid Preparation:Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency. Article Snippet: The total RNA from each sample were prepped for RNA sequencing using the New England Biolabs (NEB) Next small RNA library prep set for Illumina. .. This plasmid was linearized by incubation of 50 μg of pDNA at 37 °C for 3 h with 50 U of Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. The plasmids were digested with Purification:Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with Gel Extraction:Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with Mutagenesis:Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with Gel Purification:Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Ligation:Article Title: The genetic architecture of an allosteric hormone receptor. Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and Incubation:Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency. Article Snippet: The total RNA from each sample were prepped for RNA sequencing using the New England Biolabs (NEB) Next small RNA library prep set for Illumina. .. This plasmid was linearized by incubation of 50 μg of pDNA at 37 °C for 3 h with 50 U of Clone Assay:Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus Article Snippet: .. The plasmids were digested with Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with Construct:Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with Cloning:Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with Modification:Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with In Vitro:Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with |