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spei hf  (New England Biolabs)


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    Structured Review

    New England Biolabs spei hf
    Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spei+hf/SpeI-HF/pmc13129541-159-17-18
    Average 96 stars, based on 1132 article reviews
    spei hf - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus
    Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with SpeI-HF (NEB, R3133S) and purified the linearized plasmid via gel extraction. .. We assembled the Sox2P-mTurq-polyA fragments together with the linearized plasmid with NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S) to create the final plasmids containing FRT-3xCBS(Fw)-Sox2P-mTurq-polyA-F3 called pMK01 and FRT-3xCBS(Rv)-Sox2P-mTurq-polyA-F3 called pMK02.

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation. .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation.

    Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency.
    Article Snippet: The total RNA from each sample were prepped for RNA sequencing using the New England Biolabs (NEB) Next small RNA library prep set for Illumina. .. This plasmid was linearized by incubation of 50 μg of pDNA at 37 °C for 3 h with 50 U of SpeI-HF (NEB) enzyme in 1× reaction buffer. .. A final concentration of 12.5 pM was loaded into a MiSeq V3 kit and sequenced in a Miseq system (Illumina) for 176 total cycles.

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation81. ..

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus
    Article Snippet: .. The plasmids were digested with SpeI-HF (NEB, R3133S) and NheI-HF (NEB, R3131S) and cloned in between Sleeping Beauty arms of a target vector. .. The inserts were PCR amplified with primers containing FRT (MEP74) and F3 sites (MEP131) using NEBNext High-Fidelity 2x PCR Master Mix (NEB, M0544) and cloned into StuI digested pCRTM-Zero backbone (Addgene 120275) using the Takara ligation mix according to the manufacturers protocol.

    Purification:

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus
    Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with SpeI-HF (NEB, R3133S) and purified the linearized plasmid via gel extraction. .. We assembled the Sox2P-mTurq-polyA fragments together with the linearized plasmid with NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S) to create the final plasmids containing FRT-3xCBS(Fw)-Sox2P-mTurq-polyA-F3 called pMK01 and FRT-3xCBS(Rv)-Sox2P-mTurq-polyA-F3 called pMK02.

    Gel Extraction:

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus
    Article Snippet: .. Next, we digested the plasmid containing the FRT-3xCBS-Fw-F3 (pME038) or FRT-3xCBS-Rv-F3 (pME039) array with SpeI-HF (NEB, R3133S) and purified the linearized plasmid via gel extraction. .. We assembled the Sox2P-mTurq-polyA fragments together with the linearized plasmid with NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S) to create the final plasmids containing FRT-3xCBS(Fw)-Sox2P-mTurq-polyA-F3 called pMK01 and FRT-3xCBS(Rv)-Sox2P-mTurq-polyA-F3 called pMK02.

    Mutagenesis:

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation. .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation.

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation81. ..

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. .. Short RNA oligos for motif randomization experiments (<300 nucleotides) were transcribed from PCR products using the MEGAshortscriptTM T7 Transcription Kit (Invitrogen, AM1354).

    Gel Purification:

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation. .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation.

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation81. ..

    Ligation:

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation. .. We subcloned the four mutant PYL1 libraries into both the pMS-GluePCA1 and pMS-PYL1-PYL1 homodimer abundancePCA plasmid by BamHI-HF and SpeI-HF (New England Biolabs, Ipswich, USA; Catalog No. R3136, R3133) digestion and gel purification, followed by overnight T4 temperature-cycle ligation.

    Incubation:

    Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency.
    Article Snippet: The total RNA from each sample were prepped for RNA sequencing using the New England Biolabs (NEB) Next small RNA library prep set for Illumina. .. This plasmid was linearized by incubation of 50 μg of pDNA at 37 °C for 3 h with 50 U of SpeI-HF (NEB) enzyme in 1× reaction buffer. .. A final concentration of 12.5 pM was loaded into a MiSeq V3 kit and sequenced in a Miseq system (Illumina) for 176 total cycles.

    Clone Assay:

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus
    Article Snippet: .. The plasmids were digested with SpeI-HF (NEB, R3133S) and NheI-HF (NEB, R3131S) and cloned in between Sleeping Beauty arms of a target vector. .. The inserts were PCR amplified with primers containing FRT (MEP74) and F3 sites (MEP131) using NEBNext High-Fidelity 2x PCR Master Mix (NEB, M0544) and cloned into StuI digested pCRTM-Zero backbone (Addgene 120275) using the Takara ligation mix according to the manufacturers protocol.

    Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites
    Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with SpeI-HF (New England Biolabs R3133) and NotI (New England Biolabs R0189), and the resulting 290 bp fragment was cloned into pGEM T-easy (Promega A1360). ..

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. .. Short RNA oligos for motif randomization experiments (<300 nucleotides) were transcribed from PCR products using the MEGAshortscriptTM T7 Transcription Kit (Invitrogen, AM1354).

    Construct:

    Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites
    Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with SpeI-HF (New England Biolabs R3133) and NotI (New England Biolabs R0189), and the resulting 290 bp fragment was cloned into pGEM T-easy (Promega A1360). ..

    Cloning:

    Article Title: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites
    Article Snippet: .. To generate the hobbit C-terminal deletion constructs, the Gateway cloning entry vector pENTR1A - hobbit ( ) was digested with SpeI-HF (New England Biolabs R3133) and NotI (New England Biolabs R0189), and the resulting 290 bp fragment was cloned into pGEM T-easy (Promega A1360). ..

    Modification:

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. .. Short RNA oligos for motif randomization experiments (<300 nucleotides) were transcribed from PCR products using the MEGAshortscriptTM T7 Transcription Kit (Invitrogen, AM1354).

    In Vitro:

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Both wild-type and mutant plasmids (A-G mutation at modification site) were cloned, and plasmids were linearized with SpeI-HF (NEB, R3133S) prior to in vitro transcription. .. Short RNA oligos for motif randomization experiments (<300 nucleotides) were transcribed from PCR products using the MEGAshortscriptTM T7 Transcription Kit (Invitrogen, AM1354).



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